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Journal: Cell reports

Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis

doi: 10.1016/j.celrep.2025.115356

Figure Lengend Snippet:

Article Snippet: Following viability stain, cells were blocked in the same manner as described for CITE-seq preparation, using FcR Blocking Reagent (Miltenyi Biotec, 130-092-575) and then stained with one or a combination of the following antibodies: rat anti-mouse CD3e-647 (BioLegend, 155609, KT3.1.1), hamster anti-mouse TCR γ/δ-PE (BioLegend, 118107, GL3), rat anti-mouse CD117(c-kit)-PE-Cy7 (BioLegend, 105813, 2B8), mouse anti-mouse CX3CR1–421 (BioLegend, 149023, SA011F11), rat anti-mouse CD192(CCR2)-510 (BioLegend, 150617, SA203G11), rat anti-mouse CD3-SparkBlue550 (BioLegend, 100259, 17A2), rat anti-mouse CD8-FITC (BioLegend, 100706, 53–6.7), rat anti-mouse CD4–711 (BioLegend, 100549, RM4–5), rat anti-mouse Ly-6C-785 (BioLegend, 128041, HK1.4), rat anti-mouse Ly-6G-650 (BioLegend, 127641, 1A8), rat anti-mouse CD186(CXCR6)-PerCP/Cyanine5.5 (BioLegend, 151120, SA051D1), rat anti-mouse CD11b-605 (BioLegend, 101237, M1/70), rat anti-mouse CD45-PacificBlue (BioLegend, 103125, 30-F11), rat anti-mouse CD45-PerCP/Cyanine5.5 (BioLegend, 103131, 30-F11), rat anti-CD3-FITC (BioLegend, 100203, 17A2), hamster anti-mouse TCR γ/δ-PE-Cy7 (BioLegend, 118123, GL3), rat anti-mouse CD279(PD-1)-421 (BioLegend, 135217, 29F.1A12), rat anti-CD25-PE (BioLegend, 102007, PC61), rat anti-CD4-APC-Cy7 (Tonbo, 25-0042-U025, RM4–5), rat anti-mouse CD8a-710 (Tonbo, 80-0081-U025, 53–6.7), rat anti-mouse CD3-PacificBlue (BioLegend, 100213, 17A2), rat anti-mouse CD4–570 (BioLegend, 100541, RM4–5), rat anti-mouse CD8a-APC-Cy7 (BioLegend, 100713, 53–6.7), hamster anti-mouse TCR γ/δ−711 (BioLegend, 118149, GL3), mouse anti-mouse NK-1.1-SparkRed718 (BioLegend, 156533, S17016D), rat anti-mouse CD25–750 (BioLegend, 102077, PC61), mouse anti-mouse CD159a(NKG2A B6 )-PE (BioLegend, 142803, 16A11), rat anti-mouse CD107a(LAMP-1)-PE-Cy7 (BioLegend, 121619, 1D4B), rat anti-mouse/human CD11b-650 (BioLegend, 101259, M1/70), rat anti-mouse CD8a-647 (BioLegend, 100727, 53–6.7), rat anti-mouse CD279(PD-1)-421 (BioLegend, 135221, 29F.1A12), rat anti-mouse FOXP3–647 (BioLegend, 126407, MF-14).

Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence

Journal: Cell reports

Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis

doi: 10.1016/j.celrep.2025.115356

Figure Lengend Snippet:

Article Snippet: Flow: rat anti-CD4-APC-Cy7 (RM4–5) , Tonbo , Cat#25–0042-U025; RRID: N/A.

Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence