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Journal: Cell reports
Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis
doi: 10.1016/j.celrep.2025.115356
Figure Lengend Snippet:
Article Snippet: Following viability stain, cells were blocked in the same manner as described for CITE-seq preparation, using FcR Blocking Reagent (Miltenyi Biotec, 130-092-575) and then stained with one or a combination of the following antibodies: rat anti-mouse CD3e-647 (BioLegend, 155609, KT3.1.1), hamster anti-mouse TCR γ/δ-PE (BioLegend, 118107, GL3), rat anti-mouse CD117(c-kit)-PE-Cy7 (BioLegend, 105813, 2B8), mouse anti-mouse CX3CR1–421 (BioLegend, 149023, SA011F11), rat anti-mouse CD192(CCR2)-510 (BioLegend, 150617, SA203G11), rat anti-mouse CD3-SparkBlue550 (BioLegend, 100259, 17A2), rat anti-mouse CD8-FITC (BioLegend, 100706, 53–6.7), rat anti-mouse CD4–711 (BioLegend, 100549, RM4–5), rat anti-mouse Ly-6C-785 (BioLegend, 128041, HK1.4), rat anti-mouse Ly-6G-650 (BioLegend, 127641, 1A8), rat anti-mouse CD186(CXCR6)-PerCP/Cyanine5.5 (BioLegend, 151120, SA051D1), rat anti-mouse CD11b-605 (BioLegend, 101237, M1/70), rat anti-mouse CD45-PacificBlue (BioLegend, 103125, 30-F11), rat anti-mouse CD45-PerCP/Cyanine5.5 (BioLegend, 103131, 30-F11), rat anti-CD3-FITC (BioLegend, 100203, 17A2), hamster anti-mouse TCR γ/δ-PE-Cy7 (BioLegend, 118123, GL3), rat anti-mouse CD279(PD-1)-421 (BioLegend, 135217, 29F.1A12), rat anti-CD25-PE (BioLegend, 102007, PC61),
Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence
Journal: Cell reports
Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis
doi: 10.1016/j.celrep.2025.115356
Figure Lengend Snippet:
Article Snippet:
Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence